Free-Solution, Label-Free Molecular Interactions Studied by Back-Scattering Interferometry
نویسندگان
چکیده
منابع مشابه
Free-solution, label-free molecular interactions studied by back-scattering interferometry.
Free-solution, label-free molecular interactions were investigated with back-scattering interferometry in a simple optical train composed of a helium-neon laser, a microfluidic channel, and a position sensor. Molecular binding interactions between proteins, ions and protein, and small molecules and protein, were determined with high dynamic range dissociation constants (Kd spanning six decades)...
متن کاملLabel-free screening of bio-molecular interactions.
The majority of techniques currently employed to interrogate a biomolecular interaction require some type of radio- or enzymatic- or fluorescent-labelling to report the binding event. However, there is an increasing awareness of novel techniques that do not require labelling of the ligand or the receptor, and that allow virtually any complex to be screened with minimal assay development. This r...
متن کاملHigh-speed label-free detection by spinning-disk micro-interferometry.
Spinning-disk interferometers are a new class of analytic sensors to detect immobilized biomolecules with high speed and high sensitivity. The disks are composed of a large number of surface-normal self-referencing interferometers, analogous to an optical CD, but operating on the principle of microdiffraction quadrature that achieves sensitive linear detection of bound molecules. The surface-no...
متن کاملReply to Varma: Elucidation of the signal origin for label-free, free-solution interactions.
i) Our empirical model is informative, experimentally consistent, and a reasonable starting point for further elucidating interferometric free-solution, label-free assays. Our approach exhibits a strong correlation (Spearman’s rank) between signal magnitude with binding-induced conformation and hydration changes. This correlation held true for a diverse set of binding pairs, ranging widely in a...
متن کاملRobust analysis of synthetic label-free DNA junctions in solution by X-ray scattering and molecular simulation
Structural analysis of branched DNA molecules (BDM) is important as model systems for DNA junctions and also as building units for DNA assembly. Although there have been efforts to study the structures of BDM, label-free solution structures have not been well determined yet. Here, we used a combination of synchrotron-based experimental tools and computational simulation to study the global stru...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Science
سال: 2007
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.1146559